PGK1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PGK1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to internal region of human PGK1.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
The PGK1 polyclonal antibody (Cat # PAB2618) is used in Western blot to detect PGK1 in mouse stomach tissue lysate (Lane 1) and HepG2 cell lysate (Lane 2) .Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocarcinomareacted with PGK1 polyclonal antibody (Cat # PAB2618), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. -
Gene Info — PGK1
Entrez GeneID
5230Protein Accession#
P00558Gene Name
PGK1
Gene Alias
MGC117307, MGC142128, MGC8947, MIG10, PGKA
Gene Description
phosphoglycerate kinase 1
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. This gene lies on the X-chromosome, while a related pseudogene also has been found on the X-chromosome and another on chromosome 19. [provided by RefSeq
Other Designations
OTTHUMP00000023595|cell migration-inducing gene 10 protein|primer recognition protein 2
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Interactome
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Pathway
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Carbon fixation in photosynthetic organisms
- Glycolysis / Gluconeogenesis
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Disease
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Publication Reference
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Parkinson's disease-associated mutations in leucine-rich repeat kinase 2 augment kinase activity.
West AB, Moore DJ, Biskup S, Bugayenko A, Smith WW, Ross CA, Dawson VL, Dawson TM.
PNAS 2005 Nov; 102(46):16842.
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Parkinson's disease-associated mutations in leucine-rich repeat kinase 2 augment kinase activity.
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