PGK1 recombinant monoclonal antibody, clone R04-9H9
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human PGK1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human PGK1.
Theoretical MW (kDa)
Calculated MW: 45 kD
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1:50-1:200)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at -20 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Hela lysates with PGK1 recombinant monoclonal antibody, clone R04-9H9 (Cat # RAB02006).Western Blot
Western blot analysis of Lane 1: MCF-7, Lane 2: HCT116, Lane 3: T47D and Lane 4: Hela lysates with PGK1 recombinant monoclonal antibody, clone R04-9H9 (Cat # RAB02006).Immunofluorescence
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Gene Info — PGK1
Entrez GeneID
5230Protein Accession#
P00558Gene Name
PGK1
Gene Alias
MGC117307, MGC142128, MGC8947, MIG10, PGKA
Gene Description
phosphoglycerate kinase 1
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. This gene lies on the X-chromosome, while a related pseudogene also has been found on the X-chromosome and another on chromosome 19. [provided by RefSeq
Other Designations
OTTHUMP00000023595|cell migration-inducing gene 10 protein|primer recognition protein 2
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Interactome
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Pathway
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Carbon fixation in photosynthetic organisms
- Glycolysis / Gluconeogenesis
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