UBA2 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
The UBA2 polyclonal antibody (Cat # PAB2233) is used in Western blot to detect UBA2 in Jurkat lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with the UBA2 polyclonal antibody (Cat # PAB2233) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of UBA2.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human UBA2.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
The UBA2 polyclonal antibody (Cat # PAB2233) is used in Western blot to detect UBA2 in Jurkat lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with the UBA2 polyclonal antibody (Cat # PAB2233) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma. -
Gene Info — UBA2
Entrez GeneID
10054Protein Accession#
NP_005490Gene Name
UBA2
Gene Alias
ARX, FLJ13058, HRIHFB2115, SAE2
Gene Description
ubiquitin-like modifier activating enzyme 2
Gene Ontology
HyperlinkGene Summary
ubiquitin-activating enzyme E1 homolog|anthracycline-associated resistance
Other Designations
SUMO-1 activating enzyme subunit 2|SUMO1 activating enzyme subunit 2|UBA2, ubiquitin-activating enzyme E1 homolog|anthracycline-associated resistance
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Interactome
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Pathway
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Publication Reference
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Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1.
Desterro JM, Rodriguez MS, Kemp GD, Hay RT.
The Journal of Biological Chemistry 1999 Apr; 274(15):10618.
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Molecular cloning and characterization of human AOS1 and UBA2, components of the sentrin-activating enzyme complex.
Gong L, Li B, Millas S, Yeh ET.
FEBS Letters 1999 Apr; 448(1):185.
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In vitro SUMO-1 modification requires two enzymatic steps, E1 and E2.
Okuma T, Honda R, Ichikawa G, Tsumagari N, Yasuda H.
Biochemical and Biophysical Research Communications 1999 Jan; 254(3):693.
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Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1.
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