MRPS15 polyclonal antibody
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with MRPS15 polyclonal antibody (Cat # PAB22107) at 1:250-1:500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human duodenum with MRPS15 polyclonal antibody (Cat # PAB22107) strong cytoplasmic positivity in glandular cells at 1:20-1:50 dilution.
Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with MRPS15 polyclonal antibody (Cat # PAB22107) at 1-4 ug/mL dilution shows positivity in nuclear membrane and mitochondria.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant MRPS15.
Immunogen
Recombinant protein corresponding to amino acids of human MRPS15.
Sequence
GLGIEYTFPPLYYRRAHRRFVTKKALCIRVFQETQKLKKRRRALKAAAAAQKQAKRRNPDSPAKAIPKTLKDS
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:20-1:50)
Western Blot (1:250-1:500)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with MRPS15 polyclonal antibody (Cat # PAB22107) at 1:250-1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human duodenum with MRPS15 polyclonal antibody (Cat # PAB22107) strong cytoplasmic positivity in glandular cells at 1:20-1:50 dilution.Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with MRPS15 polyclonal antibody (Cat # PAB22107) at 1-4 ug/mL dilution shows positivity in nuclear membrane and mitochondria. -
Gene Info — MRPS15
Entrez GeneID
64960Protein Accession#
P82914Gene Name
MRPS15
Gene Alias
DC37, FLJ11564, MPR-S15, RPMS15, S15mt
Gene Description
mitochondrial ribosomal protein S15
Gene Ontology
HyperlinkGene Summary
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S15P family. The encoded protein is more than two times the size of its E. coli counterpart, with the 12S rRNA binding sites conserved. Between human and mouse, the encoded protein is the least conserved among small subunit ribosomal proteins. Pseudogenes corresponding to this gene are found on chromosomes 15q and 19q. [provided by RefSeq
Other Designations
28S ribosomal protein S15, mitochondrial|OTTHUMP00000009727
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