Western blot analysis of PDX1 (arrow) using PDX1 polyclonal antibody (Cat # PAB2101). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDX1 gene (Lane 2).
Formalin-fixed and paraffin-embedded human testis tissue reacted with PDX1 polyclonal antibody (Cat # PAB2101) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
The protein encoded by this gene is a transcriptional activator of several genes, including insulin, somatostatin, glucokinase, islet amyloid polypeptide, and glucose transporter type 2. The encoded nuclear protein is involved in the early development of the pancreas and plays a major role in glucose-dependent regulation of insulin gene expression. Defects in this gene are a cause of pancreatic agenesis, which can lead to early-onset insulin-dependent diabetes mellitus (NIDDM), as well as maturity onset diabetes of the young type 4 (MODY4). [provided by RefSeq