IPF1 monoclonal antibody (M02), clone 4E12
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of PDX1 expression in transfected 293T cell line by IPF1 monoclonal antibody (M02), clone 4E12.
Lane 1: PDX1 transfected lysate (Predicted MW: 31.13 KDa).
Lane 2: Non-transfected lysate.
Immunofluorescence
Immunofluorescence of monoclonal antibody to PDX1 on HeLa cell . [antibody concentration 10 ug/ml]
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant IPF1.
Immunogen
IPF1 (NP_000200, 109 a.a. ~ 208 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
LEEPNRVQLPFPWMKSTKAHAWKGQWAGGAYAAEPEENKRTRTAYTRAQLLELEKEFLFNKYISRPRRVELAVMLNLTERHIKIWFQNRRMKWKKEEDKK
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of PDX1 expression in transfected 293T cell line by IPF1 monoclonal antibody (M02), clone 4E12.
Lane 1: PDX1 transfected lysate (Predicted MW: 31.13 KDa).
Lane 2: Non-transfected lysate.
ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to PDX1 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — PDX1
Entrez GeneID
3651GeneBank Accession#
NM_000209Protein Accession#
NP_000200Gene Name
PDX1
Gene Alias
GSF, IDX-1, IPF1, IUF1, MODY4, PDX-1, STF-1
Gene Description
pancreatic and duodenal homeobox 1
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a transcriptional activator of several genes, including insulin, somatostatin, glucokinase, islet amyloid polypeptide, and glucose transporter type 2. The encoded nuclear protein is involved in the early development of the pancreas and plays a major role in glucose-dependent regulation of insulin gene expression. Defects in this gene are a cause of pancreatic agenesis, which can lead to early-onset insulin-dependent diabetes mellitus (NIDDM), as well as maturity onset diabetes of the young type 4 (MODY4). [provided by RefSeq
Other Designations
OTTHUMP00000018174|glucose-sensitive factor|insulin promoter factor 1, homeodomain transcription factor|insulin upstream factor 1|pancreatic-duodenal homeobox factor 1|somatostatin transcription factor 1
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Interactome
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Pathway
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Disease
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