PBK polyclonal antibody
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More Files
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Western Blot (Transfected lysate)
Western blot analysis of PBK (arrow) using PBK polyclonal antibody (Cat # PAB2035). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PBK gene (Lane 2) (Origene Technologies).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human testis tissue reacted with PBK polyclonal antibody (Cat # PAB2035) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PBK.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human PBK.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of PBK (arrow) using PBK polyclonal antibody (Cat # PAB2035). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PBK gene (Lane 2) (Origene Technologies).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human testis tissue reacted with PBK polyclonal antibody (Cat # PAB2035) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. -
Gene Info — PBK
Entrez GeneID
55872Protein Accession#
NP_060962;Q96KB5Gene Name
PBK
Gene Alias
FLJ14385, Nori-3, SPK, TOPK
Gene Description
PDZ binding kinase
Omim ID
611210Gene Ontology
HyperlinkGene Summary
This genes encodes a serine/threonine kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. This mitotic kinase may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. [provided by RefSeq
Other Designations
MAPKK-like protein kinase|PDZ-binding kinase|T-LAK cell-originated protein kinase|serine/threonine protein kinase|spermatogenesis-related protein kinase
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Interactome
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Disease
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Publication Reference
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Protein expression of PDZ-binding kinase is up-regulated in hematologic malignancies and strongly down-regulated during terminal differentiation of HL-60 leukemic cells.
Nandi A, Tidwell M, Karp J, Rapoport AP.
Blood Cells, Molecules & Diseases 2004 Jan; 32(1):240.
Application：WB-Ce, Human, AML, ALL, CLL, Burkitt's, 293 T, Human umbilical vein endothelia, HL-60 cells, Blastic type mantle cell lymphoma.
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PBK/TOPK is a novel mitotic kinase which is upregulated in Burkitt's lymphoma and other highly proliferative malignant cells.
Simons-Evelyn M, Bailey-Dell K, Toretsky JA, Ross DD, Fenton R, Kalvakolanu D, Rapoport AP.
Blood Cells, Molecules & Diseases 2001 Sep; 27(5):825.
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PDZ-binding kinase participates in spermatogenesis.
Zhao S, Dai J, Zhao W, Xia F, Zhou Z, Wang W, Gu S, Ying K, Xie Y, Mao Y.
The International Journal of Biochemistry & Cell Biology 2001 Jun; 33(6):631.
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Protein expression of PDZ-binding kinase is up-regulated in hematologic malignancies and strongly down-regulated during terminal differentiation of HL-60 leukemic cells.
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