PBK polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from COS-7 cells, treated with Nocodazole (1 ug/mL, 16 hours), using PBK polyclonal antibody (Cat # PAB18255).
Peptide "+" means "peptide blocking".
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human brain tissue using PBK polyclonal antibody (Cat # PAB18255).
Peptide "+" means "peptide blocking".
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PBK.
Immunogen
A synthetic peptide corresponding to residues surrounding T9 of human PBK.
Host
Rabbit
Reactivity
Human
Specificity
This antibody is specific to PBK.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
ELISA (1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from COS-7 cells, treated with Nocodazole (1 ug/mL, 16 hours), using PBK polyclonal antibody (Cat # PAB18255).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human brain tissue using PBK polyclonal antibody (Cat # PAB18255).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — PBK
Entrez GeneID
55872Protein Accession#
Q96KB5Gene Name
PBK
Gene Alias
FLJ14385, Nori-3, SPK, TOPK
Gene Description
PDZ binding kinase
Omim ID
611210Gene Ontology
HyperlinkGene Summary
This genes encodes a serine/threonine kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. This mitotic kinase may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. [provided by RefSeq
Other Designations
MAPKK-like protein kinase|PDZ-binding kinase|T-LAK cell-originated protein kinase|serine/threonine protein kinase|spermatogenesis-related protein kinase
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Interactome
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Disease
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Publication Reference
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PBK/TOPK, a proliferating neural progenitor-specific mitogen-activated protein kinase kinase.
Dougherty JD, Garcia AD, Nakano I, Livingstone M, Norris B, Polakiewicz R, Wexler EM, Sofroniew MV, Kornblum HI, Geschwind DH.
The Journal of Neuroscience 2005 Nov; 25(46):10773.
Application：ICC, WB-Ce, Human, Mouse, ME-180, N2A cells cells, Brain.
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PBK/TOPK, a proliferating neural progenitor-specific mitogen-activated protein kinase kinase.
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