NME3 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of NME3 polyclonal antibody (Cat # PAB2028) in CEM cell line lysate (35 ug/lane).NME3 (arrow) was detected using the purified Polyclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with NME3 polyclonal antibody (Cat # PAB2028) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of NME3.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to internal region of human NME3.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of NME3 polyclonal antibody (Cat # PAB2028) in CEM cell line lysate (35 ug/lane).NME3 (arrow) was detected using the purified Polyclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with NME3 polyclonal antibody (Cat # PAB2028) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma. -
Gene Info — NME3
Entrez GeneID
4832Protein Accession#
NP_002504;Q13232Gene Name
NME3
Gene Alias
DR-nm23, KIAA0516, NDPK-C, NDPKC, NM23-H3, c371H6.2
Gene Description
non-metastatic cells 3, protein expressed in
Omim ID
601817Gene Ontology
HyperlinkGene Summary
O
Other Designations
NDP kinase C|nucleoside-diphosphate kinase 3
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Interactome
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Pathway
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Publication Reference
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ELOVL2-AS1 suppresses tamoxifen resistance by sponging miR-1233-3p in breast cancer.
Hyeon Woo Kim, Minjae Baek, Sanghyun Jung, Siyeon Jang, Hyeonjin Lee, Seung-Hoon Yang, Beom Seok Kwak, Sun Jung Kim.
Epigenetics 2023 Dec; 18(1):2276384.
Application：WB, Human, MCF-7 cells.
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Neuroblastoma specific effects of DR-nm23 and its mutant forms on differentiation and apoptosis.
Negroni A, Venturelli D, Tanno B, Amendola R, Ransac S, Cesi V, Calabretta B, Raschella G.
Cell Death and Differentiation 2000 Sep; 7(9):843.
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Gene structure, promoter activity, and chromosomal location of the DR-nm23 gene, a related member of the nm23 gene family.
Martinez R, Venturelli D, Perrotti D, Veronese ML, Kastury K, Druck T, Huebner K, Calabretta B.
Cancer Research 1997 Mar; 57(6):1180.
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Overexpression of DR-nm23, a protein encoded by a member of the nm23 gene family, inhibits granulocyte differentiation and induces apoptosis in 32Dc13 myeloid cells.
Venturelli D, Martinez R, Melotti P, Casella I, Peschle C, Cucco C, Spampinato G, Darzynkiewicz Z, Calabretta B.
PNAS 1995 Aug; 92(16):7435.
Application：WB-Ce, WB-Tr, Mouse, 32Dc13 myeloid cells.
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ELOVL2-AS1 suppresses tamoxifen resistance by sponging miR-1233-3p in breast cancer.
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