NKX2-2 polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells with NKX2-2 polyclonal antibody (Cat # PAB20216).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human pancreas with NKX2-2 polyclonal antibody (Cat # PAB20216) shows strong nuclear positivity in islet cells.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant NKX2-2.
Immunogen
Recombinant protein corresponding to amino acids of human NKX2-2.
Sequence
SLTNTKTGFSVKDILDLPDTNDEEGSVAEGPEEENEGPEPAKRAGPLGQGALDAVQSLPLKNPFYDSSDNPYTRWLASTEGLQYSLHGLAAGAPPQDSSSKSPEPSADESPDNDKETP
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:200)
Western Blot (1:250-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells with NKX2-2 polyclonal antibody (Cat # PAB20216).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human pancreas with NKX2-2 polyclonal antibody (Cat # PAB20216) shows strong nuclear positivity in islet cells. -
Gene Info — NKX2-2
Entrez GeneID
4821Protein Accession#
O95096Gene Name
NKX2-2
Gene Alias
NKX2.2, NKX2B
Gene Description
NK2 homeobox 2
Omim ID
604612Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene contains a homeobox domain and may be involved in the morphogenesis of the central nervous system. This gene is found on chromosome 20 near NKX2-4, and these two genes appear to be duplicated on chromosome 14 in the form of TITF1 and NKX2-8. The encoded protein is likely to be a nuclear transcription factor. [provided by RefSeq
Other Designations
NK-2 homolog B|NK2 transcription factor related, locus 2|NK2 transcription factor-like protein B|OTTHUMP00000030405|homeobox protein NK-2 homolog B
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Interactome
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Pathway
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Disease
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