Rabbit polyclonal antibody raised against full length recombinant HICE1.
Recombinant GST fusion protein corresponding to full length human HICE1.
Theoretical MW (kDa):
This antibody reacts with endogenous Hice1 protein. A BLAST analysis was used to suggest reactivity with Hice1 from human based on a 100% homology with the immunizing sequence. Expect reactivity with Hice1 from chimpanzee, Sumatran orangutan based on a 90% homology with the immunizing sequence.
ELISA (1:250000) Western Blot (1:10000) The optimal working dilution should be determined by the end user.
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot analysis of S phase HeLa cells with HICE1 polyclonal antibody (Cat # PAB19929) at 1:10000 dilution. Lane 1: lysates from sh-Luciferase lentivirus-infected cells as control; Lane 2: lysates from sh-HICE1 RNAi-treated lentivirus-infected cells.