PPPDE1 polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
HEK293 lysate (10 ug protein in RIPA buffer) overexpressing human PPPDE1 with C-terminal MYC tag probed with PPPDE1 polyclonal antibody (Cat # PAB19710) (0.5 ug/mL) in lane 1 and probed with anti-MYC Tag (1/5000) in lane 3. Mock-transfected HEK293 probed with PPPDE1 polyclonal antibody (Cat # PAB19710) (1 mg/mL) in lane 2. Primary incubations were for 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of PPPDE1.
Immunogen
A synthetic peptide corresponding to internal region of human PPPDE1.
Sequence
C-ELGKEYKGNAYH
Host
Goat
Theoretical MW (kDa)
30
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Recommend Usage
ELISA (1:32000)
Western Blot (0.5-1ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
HEK293 lysate (10 ug protein in RIPA buffer) overexpressing human PPPDE1 with C-terminal MYC tag probed with PPPDE1 polyclonal antibody (Cat # PAB19710) (0.5 ug/mL) in lane 1 and probed with anti-MYC Tag (1/5000) in lane 3. Mock-transfected HEK293 probed with PPPDE1 polyclonal antibody (Cat # PAB19710) (1 mg/mL) in lane 2. Primary incubations were for 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PPPDE1
Entrez GeneID
51029Protein Accession#
NP_057160.2Gene Name
PPPDE1
Gene Alias
PNAS-4, CGI-146, FAM152A, C1orf121
Gene Description
PPPDE peptidase domain containing 1
Gene Ontology
HyperlinkOther Designations
OTTHUMP00000038197|hypothetical protein LOC51029
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