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Last updated: 2016/9/25
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SMAD4 (phospho T277) polyclonal antibody

  • Catalog # : PAB1965
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of SMAD4.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to residues surrounding T277 of human SMAD4.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein A purification
  • Recommend Usage:
  • Dot Blot (1:500)
    Immunohistochemistry (1:10-50)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Formalin-fixed and paraffin-embedded human breast cancer tissue reacted with SMAD4 (phospho T277) polyclonal antibody (Cat # PAB1965) which was peroxidase-conjugated to the secondary antibody followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • Dot Blot (Peptide)
  • Dot Blot (Peptide)
  • Dot blot analysis of SMAD4 (phospho T277) polyclonal antibody (Cat # PAB1965) on nitrocellulose membrane. 50 ug of Phospho-peptide or Non Phospho-peptide per dot were adsorbed.
  • Application Image
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
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  • Gene Information
  • Entrez GeneID:
  • 4089
  • Protein Accession#:
  • NP_005350;Q13485
  • Gene Name:
  • SMAD4
  • Gene Alias:
  • DPC4,JIP,MADH4
  • Gene Description:
  • SMAD family member 4
  • Other Designations:
  • MAD, mothers against decapentaplegic homolog 4,OTTHUMP00000163548,SMAD, mothers against DPP homolog 4,deleted in pancreatic carcinoma locus 4,mothers against decapentaplegic homolog 4,mothers against decapentaplegic, Drosophila, homolog of, 4
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