SMAD4 monoclonal antibody, clone RM277
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against of human SMAD4.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to C-terminus of human SMAD4.
Sequence
N/A
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:1000-1:2000)
Western Blotting (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: Jurkat and Lane 2: IMR32 cell lysates with SMAD4 monoclonal antibody, clone RM277 (Cat # MAB14924).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human breast cancer with SMAD4 monoclonal antibody, clone RM277 (Cat # MAB14924).Immunohistochemistry
Immunohistochemical staining of formalin fixed and paraffin embedded human colon cancer tissue sections using SMAD4 monoclonal antibody, clone RM277 (Cat# MAB14924) at a 1:5000 dilution. -
Gene Info — SMAD4
Entrez GeneID
4089Protein Accession#
Q13485Gene Name
SMAD4
Gene Alias
DPC4, JIP, MADH4
Gene Description
SMAD family member 4
Gene Ontology
HyperlinkGene Summary
mothers against decapentaplegic homolog 4|OTTHUMP00000163548|SMAD
Other Designations
MAD, mothers against decapentaplegic homolog 4|OTTHUMP00000163548|SMAD, mothers against DPP homolog 4|deleted in pancreatic carcinoma locus 4|mothers against decapentaplegic homolog 4|mothers against decapentaplegic, Drosophila, homolog of, 4
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Interactome
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Pathway
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Disease
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