DDT polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of tissue extracts with DDT polyclonal antibody (Cat # PAB19532).
Lane 1, rat brain tissue lysate.
Lane 2, rat pancreas tissue lysate.
Lane 3, rat liver tissue lysate.
Lane 4, rat lung tissue lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded human placenta specimen tissue section with DDT polyclonal antibody (Cat # PAB19532).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of DDT.
Immunogen
A synthetic peptide corresponding to amino acids at C-terminus of human DDT.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
It different from the related mouse and rat sequence by two amino acids.
Form
Lyophilized
Purification
Immunoaffinity purification
Isotype
IgG
Recommend Usage
Western Blot (1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 0.9 mg NaCl, 0.2 mg Na2HPO4 (5 mg BSA, 0.05 mg sodium azide, 0.05 mg Thimerosal)
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with 200 uL of deionized water and concentration will be 500 ug/mL, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide and thimerosal: POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of tissue extracts with DDT polyclonal antibody (Cat # PAB19532).
Lane 1, rat brain tissue lysate.
Lane 2, rat pancreas tissue lysate.
Lane 3, rat liver tissue lysate.
Lane 4, rat lung tissue lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded human placenta specimen tissue section with DDT polyclonal antibody (Cat # PAB19532). -
Gene Info — DDT
Entrez GeneID
1652Gene Name
DDT
Gene Alias
DDCT
Gene Description
D-dopachrome tautomerase
Omim ID
602750Gene Ontology
HyperlinkGene Summary
D-dopachrome tautomerase converts D-dopachrome into 5,6-dihydroxyindole. The DDT gene is related to the migration inhibitory factor (MIF) in terms of sequence, enzyme activity, and gene structure. DDT and MIF are closely linked on chromosome 22. [provided by RefSeq
Other Designations
D-dopachrome decarboxylase
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Interactome
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Disease
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Publication Reference
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Overexpression of D-dopachrome tautomerase increases ultraviolet B irradiation-induced skin tumorigenesis in mice.
Yoko Yoshihisa, Mati Ur Rehman, Tsugunobu Andoh, Yoshiaki Tabuchi, Teruhiko Makino, Tadamichi Shimizu.
FASEB Journal 2021 Jul; 35(7):e21671.
Application：IHC-P, WB-Ti, WB-Tr, Mouse, Mouse dorsal skin tumors, Mouse keratinocytes.
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Role of macrophage migration inhibitory factor in heat-induced apoptosis in keratinocytes.
Yoshihisa Y, Rehman MU, Kondo T, Shimizu T.
FASEB Journal 2016 Nov; 30(11):3870.
Application：WB-Ce, WB-Tr, Mouse, Keratinocytes.
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Overexpression of D-dopachrome tautomerase increases ultraviolet B irradiation-induced skin tumorigenesis in mice.
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