ETFB polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
ETFB polyclonal antibody (Cat # PAB19027) (0.01 ug/mL) staining of mouse liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human liver with ETFB polyclonal antibody (Cat # PAB19027) (3.8 ug/mL). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of ETFB.
Immunogen
A synthetic peptide corresponding to amino acids 231-243 at C-terminus of human ETFB.
Sequence
C-PQRTAGVKVETTE
Host
Goat
Theoretical MW (kDa)
26
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)(3-5 ug/mL)
ELISA (1:8000)
Western Blot (0.01-0.03 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
ETFB polyclonal antibody (Cat # PAB19027) (0.01 ug/mL) staining of mouse liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human liver with ETFB polyclonal antibody (Cat # PAB19027) (3.8 ug/mL). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ETFB
Entrez GeneID
2109Protein Accession#
NP_001976.1;NP_001014763.1Gene Name
ETFB
Gene Alias
FP585, MADD
Gene Description
electron-transfer-flavoprotein, beta polypeptide
Gene Ontology
HyperlinkGene Summary
This gene encodes electron-transfer-flavoprotein, beta polypeptide, which shuttles electrons between primary flavoprotein dehydrogenases involved in mitochondrial fatty acid and amino acid catabolism and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. The gene deficiencies have been implicated in type II glutaricaciduria. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq
Other Designations
electron transfer flavoprotein beta subunit|electron transfer flavoprotein beta-subunit|electron transfer flavoprotein, beta polypeptide|electron-transferring-flavoprotein, beta polypeptide
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Interactome
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Disease
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