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ETFB polyclonal antibody

  • Catalog # : PAB19027
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of ETFB.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 231-243 at C-terminus of human ETFB.
  • Sequence:
  • C-PQRTAGVKVETTE
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 26
  • Reactivity:
  • Human, Mouse, Rat
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Recommend Usage:
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)(3-5 ug/mL)
    ELISA (1:8000)
    Western Blot (0.01-0.03 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • ETFB polyclonal antibody (Cat # PAB19027) (0.01 ug/mL) staining of mouse liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining of human liver with ETFB polyclonal antibody (Cat # PAB19027) (3.8 ug/mL). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
  • ELISA
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 2109
  • Protein Accession#:
  • NP_001976.1;NP_001014763.1
  • Gene Name:
  • ETFB
  • Gene Alias:
  • FP585,MADD
  • Gene Description:
  • electron-transfer-flavoprotein, beta polypeptide
  • Gene Summary:
  • This gene encodes electron-transfer-flavoprotein, beta polypeptide, which shuttles electrons between primary flavoprotein dehydrogenases involved in mitochondrial fatty acid and amino acid catabolism and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. The gene deficiencies have been implicated in type II glutaricaciduria. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq
  • Other Designations:
  • electron transfer flavoprotein beta subunit,electron transfer flavoprotein beta-subunit,electron transfer flavoprotein, beta polypeptide,electron-transferring-flavoprotein, beta polypeptide
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