CA1 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of CA1.
Immunogen
A synthetic peptide corresponding to amino acids at internal region of human CA1.
Sequence
C-QAIKTKGKRAP
Host
Goat
Theoretical MW (kDa)
29
Reactivity
Human
Specificity
Reported variants represent identical protein: NP_001729.1, NP_001122303.1, NP_001122302.1, NP_001122301.1.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:64000)
Western Blot (0.03-0.1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
CA1 polyclonal antibody (Cat # PAB18720) (0.03 ug/mL) staining of human liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — CA1
Entrez GeneID
759Protein Accession#
NP_001729.1Gene Name
CA1
Gene Alias
Car1
Gene Description
carbonic anhydrase I
Omim ID
114800Gene Ontology
HyperlinkGene Summary
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA1 is closely linked to CA2 and CA3 genes on chromosome 8, and it encodes a cytosolic protein which is found at the highest level in erythrocytes. Variants of this gene have been described in some populations. Multiple alternatively spliced variants, encoding the same protein, have been identified. Transcript variants of CA1 utilizing alternative polyA_sites have been described in literature. [provided by RefSeq
Other Designations
carbonic dehydratase
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Interactome
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Pathway
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Disease
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