CA1 recombinant monoclonal antibody, clone 26E3
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human CA1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human CA1.
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography purification
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry(1:50-1:200)
Immunohistochemistry(1:50-1:200)
Western Blot(1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150 mM NaCl, 0.02% sodium azide and 50% glycerol)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: K562 whole cell lysate; Lane 2: U937 whole cell lysate.Immunohistochemistry
Immunohistochemistry image of CA1 recombinant monoclonal antibody, clone 26E3 diluted at 1:50 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Overlay Peak curve showing MCF-7 cells stained with CA1 recombinant monoclonal antibody, clone 26E3 (red line) at 1:50. -
Gene Info — CA1
Entrez GeneID
759Protein Accession#
P00915Gene Name
CA1
Gene Alias
Car1
Gene Description
carbonic anhydrase I
Omim ID
114800Gene Ontology
HyperlinkGene Summary
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA1 is closely linked to CA2 and CA3 genes on chromosome 8, and it encodes a cytosolic protein which is found at the highest level in erythrocytes. Variants of this gene have been described in some populations. Multiple alternatively spliced variants, encoding the same protein, have been identified. Transcript variants of CA1 utilizing alternative polyA_sites have been described in literature. [provided by RefSeq
Other Designations
carbonic dehydratase
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Interactome
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Pathway
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Disease
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