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Last updated: 2016/12/4
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NFATC2 polyclonal antibody

  • Catalog # : PAB18714
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of NFATC2.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids at internal region of human NFATC2.
  • Sequence:
  • C-QQFRTGLSSPDARYQ
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 110
  • Reactivity:
  • Human
  • Specificity:
  • This antibody is expected to recognize all three reported isoforms (NP_036472.2; NP_775114.1; NP_001129493.1).
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Recommend Usage:
  • ELISA (1:64000)
    Western Blot (0.3-1 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (3-6 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • NFATC2 polyclonal antibody (Cat # PAB18714) (0.3 ug/mL) staining of Molt 4 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • NFATC2 polyclonal antibody (Cat # PAB18714)(3.8 ug/mL) staining of paraffin embedded human spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 4773
  • Protein Accession#:
  • NP_036472.2;NP_775114.1;NP_001129493.1
  • Gene Name:
  • NFATC2
  • Gene Alias:
  • KIAA0611,NFAT1,NFATP
  • Gene Description:
  • nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2
  • Gene Summary:
  • This gene is a member of the nuclear factor of activated T cells (NFAT) family. The product of this gene is a DNA-binding protein with a REL-homology region (RHR) and an NFAT-homology region (NHR). This protein is present in the cytosol and only translocates to the nucleus upon T cell receptor (TCR) stimulation, where it becomes a member of the nuclear factors of activated T cells transcription complex. This complex plays a central role in inducing gene transcription during the immune response. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
  • Other Designations:
  • NFAT pre-existing subunit,NFAT transcription complex, preexisting component,OTTHUMP00000031291,T cell transcription factor NFAT1,nuclear factor of activated T-cells, cytoplasmic 2,nuclear factor of activated T-cells, preexisting component,preexisting nucl
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