ABCF1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of HepG2 (Lane 1) and 293T (Lane 2) and HeLa cell lysates with ABCF1 polyclonal antibody (Cat # PAB18535) at 1 : 500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human fetal colon showing cytoplasmic staining with ABCF1 polyclonal antibody (Cat # PAB18535) at a 1 : 100 dilution.
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant ABCF1.
Immunogen
Recombinant protein corresponding to amino acids 580-837 of human ABCF1.
Host
Rabbit
Reactivity
Human
Specificity
This antibody is specific to ABCF1.
Form
Liquid
Purification
Protein A purification
Recommend Usage
Western Blot (1:1000-1:2000)
Immunohistochemistry (1:100-1:500)
ELISA (1:20000-1:80000)
The optimal working dilution should be determined by the end user.Storage Buffer
In buffer containing 0.02% sodium azide
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of HepG2 (Lane 1) and 293T (Lane 2) and HeLa cell lysates with ABCF1 polyclonal antibody (Cat # PAB18535) at 1 : 500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human fetal colon showing cytoplasmic staining with ABCF1 polyclonal antibody (Cat # PAB18535) at a 1 : 100 dilution.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ABCF1
Entrez GeneID
23GeneBank Accession#
BC034488Gene Name
ABCF1
Gene Alias
ABC27, ABC50
Gene Description
ATP-binding cassette, sub-family F (GCN20), member 1
Omim ID
603429Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the GCN20 subfamily. Unlike other members of the superfamily, this protein lacks the transmembrane domains which are characteristic of most ABC transporters. This protein may be regulated by tumor necrosis factor-alpha and play a role in enhancement of protein synthesis and the inflammation process. [provided by RefSeq
Other Designations
ATP-binding cassette 50 (TNF-alpha stimulated)|ATP-binding cassette, sub-family F, member 1|OTTHUMP00000029110|OTTHUMP00000029111|TNFalpha-inducible ATP-binding protein
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Interactome
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Disease
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Publication Reference
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Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
Strausberg RL, Feingold EA, Grouse LH, Derge JG, Klausner RD, Collins FS, Wagner L, Shenmen CM, Schuler GD, Altschul SF, Zeeberg B, Buetow KH, Schaefer CF, Bhat NK, Hopkins RF, Jordan H, Moore T, Max SI, Wang J, Hsieh F, Diatchenko L, Marusina K, Farmer AA, Rubin GM, Hong L, Stapleton M, Soares MB, Bonaldo MF, Casavant TL, Scheetz TE, Brownstein MJ, Usdin TB, Toshiyuki S, Carninci P, Prange C, Raha SS, Loquellano NA, Peters GJ, Abramson RD, Mullahy SJ, Bosak SA, McEwan PJ, McKernan KJ, Malek JA,
PNAS 2002 Dec; 99(26):16899.
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Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
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