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Last updated: 2017/8/13
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PIAS1 polyclonal antibody

  • Catalog # : PAB1850
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of PIAS1.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human PIAS1.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Recommend Usage:
  • Western Blot (1:1000)
    Immunohistochemistry (1:50-100)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • The PIAS1 polyclonal antibody (Cat # PAB1850) is used in Western blot to detect PIAS1 in HL-60 tissue lysate.
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot analysis of PIAS1 (arrow) using PIAS1 polyclonal antibody (Cat # PAB1850). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PIAS1 gene (Lane 2) (Origene Technologies).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with PIAS1 polyclonal antibody (Cat # PAB1850), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 8554
  • Protein Accession#:
  • NP_057250;O75925
  • Gene Name:
  • PIAS1
  • Gene Alias:
  • DDXBP1,GBP,GU/RH-II,MGC141878,MGC141879,ZMIZ3
  • Gene Description:
  • protein inhibitor of activated STAT, 1
  • Gene Summary:
  • This gene encodes a member of the mammalian PIAS [protein inhibitor of activated STAT-1 (signal transducer and activator of transcription-1)] family. This member contains a putative zinc-binding motif and a highly acidic region. It inhibits STAT1-mediated gene activation and the DNA binding activity, binds to Gu protein/RNA helicase II/DEAD box polypeptide 21, and interacts with androgen receptor (AR). It functions in testis as a nuclear receptor transcriptional coregulator and may have a role in AR initiation and maintenance of spermatogenesis. [provided by RefSeq
  • Other Designations:
  • AR interacting protein,DEAD/H (Asp-Glu-Ala-Asp/His) box binding protein 1,protein inhibitor of activated STAT-1,zinc finger, MIZ-type containing 3
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