EIF2S1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from K-562 cells untreated or treated with IFN-a (1000 U/mL, 18 hours), using EIF2S1 polyclonal antibody (Cat # PAB18469).
Peptide "+" means "peptide blocking".
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using EIF2S1 polyclonal antibody (Cat # PAB18469).
Peptide "+" means "peptide blocking".
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of EIF2S1.
Immunogen
A synthetic peptide corresponding to residues surrounding S51 of human EIF2S1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to EIF2S1.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
ELISA (1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from K-562 cells untreated or treated with IFN-a (1000 U/mL, 18 hours), using EIF2S1 polyclonal antibody (Cat # PAB18469).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using EIF2S1 polyclonal antibody (Cat # PAB18469).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — EIF2S1
Entrez GeneID
1965Protein Accession#
P05198Gene Name
EIF2S1
Gene Alias
EIF-2, EIF-2A, EIF-2alpha, EIF2, EIF2A
Gene Description
eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
Omim ID
603907Gene Ontology
HyperlinkGene Summary
The translation initiation factor eIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, eIF2, and GTP. eIF2 is composed of 3 nonidentical subunits, alpha (36 kD), beta (38 kD, MIM 603908), and gamma (52 kD, MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of eIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM
Other Designations
eIF-2-alpha|eukaryotic translation initiation factor 2, subunit 1 (alpha, 35kD )
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Interactome
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Disease
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Publication Reference
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Multifactorial contributions to an acute DNA damage response by BRCA1/BARD1-containing complexes.
Greenberg RA, Sobhian B, Pathania S, Cantor SB, Nakatani Y, Livingston DM.
Genes & Development 2006 Jan; 20(1):34.
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BRCA1 is required for common-fragile-site stability via its G2/M checkpoint function.
Arlt MF, Xu B, Durkin SG, Casper AM, Kastan MB, Glover TW.
Molecular and Cellular Biology 2004 Aug; 24(15):6701.
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Chk2 phosphorylation of BRCA1 regulates DNA double-strand break repair.
Zhang J, Willers H, Feng Z, Ghosh JC, Kim S, Weaver DT, Chung JH, Powell SN, Xia F.
Molecular and Cellular Biology 2004 Jan; 24(2):708.
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Involvement of the cohesin protein, Smc1, in Atm-dependent and independent responses to DNA damage.
Kim ST, Xu B, Kastan MB.
Genes & Development 2002 Mar; 16(5):560.
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Functional interactions between BRCA1 and the checkpoint kinase ATR during genotoxic stress.
Tibbetts RS, Cortez D, Brumbaugh KM, Scully R, Livingston D, Elledge SJ, Abraham RT.
Genes & Development 2000 Dec; 14(23):2989.
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Multifactorial contributions to an acute DNA damage response by BRCA1/BARD1-containing complexes.
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