NCF4 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of NCF4.
Immunogen
A synthetic peptide corresponding to human NCF4.
Host
Rabbit
Reactivity
Human, Mouse
Specificity
This antibody is specific to NCF4.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Immunohistochemistry (1:50-1:100)
ELISA (1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human ovary tissue using NCF4 polyclonal antibody (Cat # PAB18253).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — NCF4
Entrez GeneID
4689Protein Accession#
Q15080Gene Name
NCF4
Gene Alias
MGC3810, NCF, P40PHOX, SH3PXD4
Gene Description
neutrophil cytosolic factor 4, 40kDa
Omim ID
601488Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq
Other Designations
OTTHUMP00000028736|OTTHUMP00000043756|neutrophil NADPH oxidase factor 4|neutrophil cytosol factor 4|neutrophil cytosolic factor 4
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Interactome
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Pathway
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Publication Reference
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Vav proteins in neutrophils are required for FcgammaR-mediated signaling to Rac GTPases and nicotinamide adenine dinucleotide phosphate oxidase component p40(phox).
Utomo A, Cullere X, Glogauer M, Swat W, Mayadas TN.
Journal of Immunology 2006 Nov; 177(9):6388.
Application:WB, Mouse, Mouse neutrophils.
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Reconstitution of chemotactic peptide-induced nicotinamide adenine dinucleotide phosphate (reduced) oxidase activation in transgenic COS-phox cells.
He R, Nanamori M, Sang H, Yin H, Dinauer MC, Ye RD.
The Journal of Immunology 2004 Dec; 173(12):7462.
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Multiple PU.1 sites cooperate in the regulation of p40(phox) transcription during granulocytic differentiation of myeloid cells.
Li SL, Valente AJ, Qiang M, Schlegel W, Gamez M, Clark RA.
Blood 2002 Jun; 99(12):4578.
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Vav proteins in neutrophils are required for FcgammaR-mediated signaling to Rac GTPases and nicotinamide adenine dinucleotide phosphate oxidase component p40(phox).
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