NCF4 recombinant monoclonal antibody, clone R02-3G7
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More Files
- More Functions
Western Blot
Western Blot analysis of HL-60, U251 lysates with NCF4 recombinant monoclonal antibody, clone R02-3G7 (Cat # RAB01390).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human tonsil with NCF4 recombinant monoclonal antibody, clone R02-3G7 (Cat # RAB01390).
Immunocytochemistry
Immunocytochemistry staining of HeLa cells with NCF4 recombinant monoclonal antibody, clone R02-3G7 (Cat # RAB01390).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human NCF4.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to human NCF4.
Theoretical MW (kDa)
Calculated MW: 39 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry
Immunofluorescence
Immunohistochemistry (Frozen sections)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoprecipitation
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In 50mM Tris-Glycine, pH 7.4, (0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of HL-60, U251 lysates with NCF4 recombinant monoclonal antibody, clone R02-3G7 (Cat # RAB01390).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human tonsil with NCF4 recombinant monoclonal antibody, clone R02-3G7 (Cat # RAB01390).Immunohistochemistry (Frozen sections)
Immunocytochemistry
Immunocytochemistry staining of HeLa cells with NCF4 recombinant monoclonal antibody, clone R02-3G7 (Cat # RAB01390).Immunofluorescence
Immunoprecipitation
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Gene Info — NCF4
Entrez GeneID
4689Protein Accession#
Q15080Gene Name
NCF4
Gene Alias
MGC3810, NCF, P40PHOX, SH3PXD4
Gene Description
neutrophil cytosolic factor 4, 40kDa
Omim ID
601488Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq
Other Designations
OTTHUMP00000028736|OTTHUMP00000043756|neutrophil NADPH oxidase factor 4|neutrophil cytosol factor 4|neutrophil cytosolic factor 4
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Interactome
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Pathway
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Disease
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