SF1 polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
-
Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of SF1.
Immunogen
A synthetic peptide corresponding to residues surrounding S82 of human SF1.
Host
Rabbit
Reactivity
Human, Mouse
Specificity
This antibody is specific to SF1.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:500-1:1000)
ELISA (1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from COLO 205 cells, using SF1 polyclonal antibody (Cat # PAB18220).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human brain tissue using SF1 polyclonal antibody (Cat # PAB18220).
Peptide "+" means "peptide blocking".Immunofluorescence
Immunofluorescence analysis of HeLa cells, using SF1 polyclonal antibody (Cat # PAB18220).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
-
Gene Info — SF1
-
Interactomes
-
Publication Reference
-
Nuclear receptors Sf1 and Dax1 function cooperatively to mediate somatic cell differentiation during testis development.
Park SY, Meeks JJ, Raverot G, Pfaff LE, Weiss J, Hammer GD, Jameson JL.
Development 2005 May; 132(10):2415.
-
The Wilms tumor suppressor WT1 regulates early gonad development by activation of Sf1.
Wilhelm D, Englert C.
Genes & Development 2002 Jul; 16(14):1839.
Application:IHC, Mouse, Embryos.
-
PNRC: a proline-rich nuclear receptor coregulatory protein that modulates transcriptional activation of multiple nuclear receptors including orphan receptors SF1 (steroidogenic factor 1) and ERRalpha1 (estrogen related receptor alpha-1).
Zhou D, Quach KM, Yang C, Lee SY, Pohajdak B, Chen S.
Molecular Endocrinology 2000 Jul; 14(7):986.
-
Nuclear receptors Sf1 and Dax1 function cooperatively to mediate somatic cell differentiation during testis development.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com