PECAM1 polyclonal antibody
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More Files
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Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PECAM1.
Immunogen
A synthetic peptide corresponding to residues surrounding Y713 of human PECAM1.
Host
Rabbit
Reactivity
Human, Mouse
Specificity
This antibody is specific to PECAM1.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:500-1:1000)
ELISA (1:20000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from Jurkat cells, using PECAM1 polyclonal antibody (Cat # PAB18207).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human brain tissue using PECAM1 polyclonal antibody (Cat # PAB18207).
Peptide "+" means "peptide blocking".Immunofluorescence
Immunofluorescence analysis of HeLa cells, using PECAM1 polyclonal antibody (Cat # PAB18207).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — PECAM1
Entrez GeneID
5175Protein Accession#
P16284Gene Name
PECAM1
Gene Alias
CD31, PECAM-1
Gene Description
platelet/endothelial cell adhesion molecule
Omim ID
173445Gene Ontology
HyperlinkGene Summary
CD31/EndoCAM|adhesion molecule
Other Designations
CD31 antigen|CD31/EndoCAM|PECAM-1, CD31/EndoCAM|adhesion molecule
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Interactomes
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Pathways
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Diseases
- Acute Disease
- Angina Pectoris
- Arteriosclerosis
- Asthma
- Astrocytoma
- Atherosclerosis
- Brain Ischemia
- Bronchiolitis
- Cadaver
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Publication Reference
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Overlapping and distinct biological effects of IL-6 classic and trans-signaling in vascular endothelial cells.
Ashani Montgomery, Franklin Tam, Chris Gursche, Catherine Cheneval, Katrina Besler, Winnie Enns, Sukhbir Manku, Kevin Rey, Paul J Hanson, Stefan Rose-John, Bruce M McManus, Jonathan C Choy.
American Journal of Physiology. Cell Physiology 2021 Apr; 320(4):C554.
Application:IHC-P, Human, Mouse, Human coronary artery samples, Mouse allograft arteries.
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PECAM-1 shedding during apoptosis generates a membrane-anchored truncated molecule with unique signaling characteristics.
Ilan N, Mohsenin A, Cheung L, Madri JA.
FASEB Journal 2001 Feb; 15(2):362.
Application:Flow Cyt, IP, WB-Ce, WB-Tr, Human, Mouse, EOMA, 293T, BAEC, Scp2, SW480 cells.
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Integrin activation by regulated dimerization and oligomerization of platelet endothelial cell adhesion molecule (PECAM)-1 from within the cell.
Zhao T, Newman PJ.
The Journal of Cell Biology 2001 Jan; 152(1):65.
Application:IF, WB-Ce, WB-Tr, Human, HEL, HEK 293 cells.
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Overlapping and distinct biological effects of IL-6 classic and trans-signaling in vascular endothelial cells.
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