PECAM1 recombinant monoclonal antibody
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human PECAM1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide of human CD31.
Theoretical MW (kDa)
130
Reactivity
Human
Specificity
Recognizes endogenous levels of CD31 protein.
Form
Liquid
Purification
Immunogen affinity chromatography
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50mM Tris-Glycine, pH 7.4 (0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Jurkat (A) whole cell lysates with CD31 recombinant monoclonal antibody (Cat # RAB02481).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of human lung cancer formalin fixed paraffin embedded tissue section using CD31 recombinant monoclonal antibody (Cat # RAB02481). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.77). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. -
Gene Info — PECAM1
Entrez GeneID
5175Protein Accession#
P16284Gene Name
PECAM1
Gene Alias
CD31, PECAM-1
Gene Description
platelet/endothelial cell adhesion molecule
Omim ID
173445Gene Ontology
HyperlinkGene Summary
CD31/EndoCAM|adhesion molecule
Other Designations
CD31 antigen|CD31/EndoCAM|PECAM-1, CD31/EndoCAM|adhesion molecule
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Interactome
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Pathway
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Disease
- Acute Disease
- Angina Pectoris
- Arteriosclerosis
- Asthma
- Astrocytoma
- Atherosclerosis
- Brain Ischemia
- Bronchiolitis
- Cadaver
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