LCK polyclonal antibody
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Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of LCK.
Immunogen
A synthetic peptide corresponding to human LCK.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to LCK.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunofluorescence (1:100-1:500)
ELISA (1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from Jurkat cells, using LCK polyclonal antibody (Cat # PAB18186).
Peptide "+" means "peptide blocking".Immunofluorescence
Immunofluorescence analysis of COS-7 cells, using LCK polyclonal antibody (Cat # PAB18186).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — LCK
Entrez GeneID
3932Protein Accession#
P06239Gene Name
LCK
Gene Alias
YT16, p56lck, pp58lck
Gene Description
lymphocyte-specific protein tyrosine kinase
Omim ID
153390Gene Ontology
HyperlinkGene Summary
This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. Multiple alternatively spliced variants, encoding the same protein, have been described. [provided by RefSeq
Other Designations
T-lymphocyte specific protein tyrosine kinase p56lck|p56(LSTRA) protein-tyrosine kinase|protein tyrosine kinase|proto-oncogene tyrosine-protein kinase LCK
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Interactomes
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Pathways
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Diseases
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Publication Reference
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Differential requirement for Lck during primary and memory CD8+ T cell responses.
Tewari K, Walent J, Svaren J, Zamoyska R, Suresh M.
PNAS 2006 Oct; 103(44):16388.
Application:WB-Ce, Mouse, Mouse T cells.
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Lck is required for stromal cell-derived factor 1 alpha (CXCL12)-induced lymphoid cell chemotaxis.
Inngjerdingen M, Torgersen KM, Maghazachi AA.
Blood 2002 Jun; 99(12):4318.
Application:IP, KA, WB-Tr, Human, JCaM1.6, NK, T cells.
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Differential requirement for Lck during primary and memory CD8+ T cell responses.
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