LCK recombinant monoclonal antibody, clone R01-8A5
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More Files
- More Functions
Western Blot
Western Blot analysis of Jurkat lysates with LCK recombinant monoclonal antibody, clone R01-8A5 (Cat # RAB02136).
Immunocytochemistry
Immunocytochemical staining of Jurkat with LCK recombinant monoclonal antibody, clone R01-8A5 (Cat # RAB02136). (A) Lck (green) and (B) DAPI (blue).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human LCK.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to human LCK.
Theoretical MW (kDa)
Calculated MW: 58 kD
Reactivity
Human, Rat
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry
Immunofluorescence(1:50-1:200)
Immunohistochemistry (1:50-1:100)
Immunoprecipitation(1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at -20 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Jurkat lysates with LCK recombinant monoclonal antibody, clone R01-8A5 (Cat # RAB02136).Immunohistochemistry
Immunocytochemistry
Immunocytochemical staining of Jurkat with LCK recombinant monoclonal antibody, clone R01-8A5 (Cat # RAB02136). (A) Lck (green) and (B) DAPI (blue).Immunofluorescence
Immunoprecipitation
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Gene Info — LCK
Entrez GeneID
3932Protein Accession#
P06239Gene Name
LCK
Gene Alias
YT16, p56lck, pp58lck
Gene Description
lymphocyte-specific protein tyrosine kinase
Omim ID
153390Gene Ontology
HyperlinkGene Summary
This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. Multiple alternatively spliced variants, encoding the same protein, have been described. [provided by RefSeq
Other Designations
T-lymphocyte specific protein tyrosine kinase p56lck|p56(LSTRA) protein-tyrosine kinase|protein tyrosine kinase|proto-oncogene tyrosine-protein kinase LCK
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Interactome
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Pathway
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Disease
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