IKBKB polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of IKBKB.
Immunogen
A synthetic peptide corresponding to human IKBKB.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to IKBKB.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
ELISA (1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from 293 cells, treated with LPS (100 ng/mL, 30 mins), using IKBKB polyclonal antibody (Cat # PAB18155).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IKBKB polyclonal antibody (Cat # PAB18155).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — IKBKB
Entrez GeneID
3551Protein Accession#
O14920Gene Name
IKBKB
Gene Alias
FLJ40509, IKK-beta, IKK2, IKKB, MGC131801, NFKBIKB
Gene Description
inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta
Omim ID
603258Gene Ontology
HyperlinkGene Summary
NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008, or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM
Other Designations
inhibitor of nuclear factor kappa B kinase beta subunit|nuclear factor NF-kappa-B inhibitor kinase beta
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Interactome
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Pathway
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Disease
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Publication Reference
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NIBP, a novel NIK and IKK(beta)-binding protein that enhances NF-(kappa)B activation.
Hu WH, Pendergast JS, Mo XM, Brambilla R, Bracchi-Ricard V, Li F, Walters WM, Blits B, He L, Schaal SM, Bethea JR.
The Journal of Biological Chemistry 2005 Aug; 280(32):29233.
Application:WB, Human, Mouse, Rat, HEK 293T cells, Mouse brains, PC-12 cells.
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IKK beta plays an essential role in the phosphorylation of RelA/p65 on serine 536 induced by lipopolysaccharide.
Yang F, Tang E, Guan K, Wang CY.
Journal of Immunology 2003 Jun; 170(11):5630.
Application:WB, Mouse, MEFs.
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IKK beta is required for Bcl-2-mediated NF-kappa B activation in ventricular myocytes.
Regula KM, Ens K, Kirshenbaum LA.
The Journal of Biological Chemistry 2002 Oct; 277(41):38676.
Application:IP, Human, 293 cells.
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NIBP, a novel NIK and IKK(beta)-binding protein that enhances NF-(kappa)B activation.
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