CCND1 polyclonal antibody
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More Files
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CCND1.
Immunogen
A synthetic peptide corresponding to human CCND1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to CCND1.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
ELISA (1:40000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from Jurkat cells, treated with EGF (200 ng/mL, 30 mins), using CCND1 polyclonal antibody (Cat # PAB18149).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human brain tissue using CCND1 polyclonal antibody (Cat # PAB18149).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — CCND1
Entrez GeneID
595Protein Accession#
P24385Gene Name
CCND1
Gene Alias
BCL1, D11S287E, PRAD1, U21B31
Gene Description
cyclin D1
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. [provided by RefSeq
Other Designations
B-cell CLL/lymphoma 1|G1/S-specific cyclin D1
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Interactome
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Pathway
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Disease
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Publication Reference
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Cyclin D1 overexpression and response to bortezomib treatment in a breast cancer model.
Ishii Y, Pirkmaier A, Alvarez JV, Frank DA, Keselman I, Logothetis D, Mandeli J, O'Connell MJ, Waxman S, Germain D.
Journal of the National Cancer Institute 2006 Sep; 98(17):1238.
Application:IP, WB, Human, HBL100, T47D cells.
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Cyclin D1 determines mitochondrial function in vivo.
Sakamaki T, Casimiro MC, Ju X, Quong AA, Katiyar S, Liu M, Jiao X, Li A, Zhang X, Lu Y, Wang C, Byers S, Nicholson R, Link T, Shemluck M, Yang J, Fricke ST, Novikoff PM, Papanikolaou A, Arnold A, Albanese C, Pestell R.
Molecular Biology of the Cell 2006 Jul; 26(14):5449.
Application:WB, Human, Mouse, MCF-7, MCF-10A cells, Mouse mammary epithelium.
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Cyclin D1 regulates cellular migration through the inhibition of thrombospondin 1 and ROCK signaling.
Li Z, Wang C, Jiao X, Lu Y, Fu M, Quong AA, Dye C, Yang J, Dai M, Ju X, Zhang X, Li A, Burbelo P, Stanley ER, Pestell RG.
Molecular and Cellular Biology 2006 Jun; 26(11):4240.
Application:WB-Ce, Mouse, MEFs.
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Cyclin D1 overexpression and response to bortezomib treatment in a breast cancer model.
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