HIST1H2BH polyclonal antibody
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Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of HIST1H2BH.
Immunogen
A synthetic peptide corresponding to internal of human HIST1H2BH.
Host
Rabbit
Reactivity
Human, Mouse
Specificity
This antibody detects endogenous levels of total HIST1H2BH protein.
Form
Liquid
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:500-1:1000)
ELISA (1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (150mM NaCl, 0.02% sodium azide, 50% glycerol)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from A-549 cells, using HIST1H2BH polyclonal antibody (Cat # PAB17428).
Peptide "+" means "with peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry analysis of paraffin-embedded human brain tissue using HIST1H2BH polyclonal antibody (Cat # PAB17428).
Peptide "+" means "with peptide blocking".Immunofluorescence
Immunofluorescence analysis of HeLa cells, using HIST1H2BH polyclonal antibody (Cat # PAB17428).
Peptide "+" means "with peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — HIST1H2BH
Entrez GeneID
8345Protein Accession#
Q93079Gene Name
HIST1H2BH
Gene Alias
H2B/j, H2BFJ
Gene Description
histone cluster 1, H2bh
Omim ID
602806Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H2B family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq
Other Designations
H2B histone family, member J|histone 1, H2bh
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