LRAT polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from COLO 205 cells, using LRAT polyclonal antibody (Cat # PAB17348).
Peptide "+" means "with peptide blocking".
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry analysis of paraffin-embedded human prostate carcinoma tissue using LRAT polyclonal antibody (Cat # PAB17348).
Peptide "+" means "with peptide blocking".
Immunofluorescence
Immunofluorescence analysis of HUVEC cells, using LRAT polyclonal antibody (Cat # PAB17348).
Peptide "+" means "with peptide blocking".
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of LRAT.
Immunogen
A synthetic peptide corresponding to internal of human LRAT.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of total LRAT protein.
Form
Liquid
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:500-1:1000)
ELISA (1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (150mM NaCl, 0.02% sodium azide, 50% glycerol)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from COLO 205 cells, using LRAT polyclonal antibody (Cat # PAB17348).
Peptide "+" means "with peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry analysis of paraffin-embedded human prostate carcinoma tissue using LRAT polyclonal antibody (Cat # PAB17348).
Peptide "+" means "with peptide blocking".Immunofluorescence
Immunofluorescence analysis of HUVEC cells, using LRAT polyclonal antibody (Cat # PAB17348).
Peptide "+" means "with peptide blocking". -
Gene Info — LRAT
Entrez GeneID
9227Protein Accession#
O95237Gene Name
LRAT
Gene Alias
MGC33103
Gene Description
lecithin retinol acyltransferase (phosphatidylcholine--retinol O-acyltransferase)
Omim ID
604863Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a microsomal enzyme that catalyzes the esterification of all-trans-retinol into all-trans-retinyl ester, an essential reaction for the retinoid cycle in visual system and vitamin A status in liver. Mutations in this gene have been associated with early-onset severe retinal dystrophy. [provided by RefSeq
Other Designations
lecithin retinol acyltransferase
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Interactome
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Pathway
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Disease
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Publication Reference
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Mutations in the gene encoding lecithin retinol acyltransferase are associated with early-onset severe retinal dystrophy.
Thompson DA, Li Y, McHenry CL, Carlson TJ, Ding X, Sieving PA, Apfelstedt-Sylla E, Gal A.
Nature Genetics 2001 Jun; 28(2):123.
Application：WB-Tr, Human, COS-7 cells.
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Lecithin retinol acyltransferase contains cysteine residues essential for catalysis.
Mondal MS, Ruiz A, Bok D, Rando RR.
Biochemistry 2000 May; 39(17):5215.
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Molecular and biochemical characterization of lecithin retinol acyltransferase.
Ruiz A, Winston A, Lim YH, Gilbert BA, Rando RR, Bok D.
The Journal of Biological Chemistry 1999 Feb; 274(6):3834.
Application：WB-Tr, Human, HEK 293T cells.
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Mutations in the gene encoding lecithin retinol acyltransferase are associated with early-onset severe retinal dystrophy.
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