Histone H3 polyclonal antibody
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Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Histone H3.
Immunogen
A synthetic peptide corresponding to internal of human Histone H3.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of total Histone H3 protein.
Form
Liquid
Recommend Usage
Immunohistochemistry (1:50-1:100)
ELISA (1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (150mM NaCl, 0.02% sodium azide, 50% glycerol)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry analysis of paraffin-embedded human brain tissue using Histone H3 polyclonal antibody (Cat # PAB17295).
Peptide "+" means "with peptide blocking". -
Publication Reference
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Isolation and characterization of proteins associated with histone H3 tails in vivo.
Heo K, Kim B, Kim K, Choi J, Kim H, Zhan Y, Ranish JA, An W.
The Journal of Biological Chemistry 2007 Apr; 282(21):15476.
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Identification and characterization of the human Set1B histone H3-Lys4 methyltransferase complex.
Lee JH, Tate CM, You JS, Skalnik DG.
The Journal of Biological Chemistry 2007 Mar; 282(18):13419.
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Isolation and characterization of proteins associated with histone H3 tails in vivo.
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