AKT1S1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from 293 cells, using AKT1S1 polyclonal antibody (Cat # PAB17288).
Peptide "+" means "with peptide blocking".
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry analysis of paraffin-embedded human brain tissue using AKT1S1 polyclonal antibody (Cat # PAB17288).
Peptide "+" means "with peptide blocking".
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of AKT1S1.
Immunogen
A synthetic peptide corresponding to residues surrounding serine 363 of human AKT1S1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of total AKT1S1 protein.
Form
Liquid
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
ELISA (1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (150mM NaCl, 0.02% sodium azide, 50% glycerol)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from 293 cells, using AKT1S1 polyclonal antibody (Cat # PAB17288).
Peptide "+" means "with peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry analysis of paraffin-embedded human brain tissue using AKT1S1 polyclonal antibody (Cat # PAB17288).
Peptide "+" means "with peptide blocking". -
Gene Info — AKT1S1
Entrez GeneID
84335Protein Accession#
Q96B36Gene Name
AKT1S1
Gene Alias
Lobe, MGC2865, PRAS40
Gene Description
AKT1 substrate 1 (proline-rich)
Omim ID
610221Gene Ontology
HyperlinkGene Summary
AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[supplied by OMIM
Other Designations
OTTHUMP00000196757|OTTHUMP00000196758|OTTHUMP00000196759|OTTHUMP00000196760|proline-rich Akt substrate, 40 kDa
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Interactome
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Publication Reference
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Gender difference of androgen actions on skeletal muscle transcriptome.
Yoshioka M, Boivin A, Bolduc C, St-Amand J.
Journal of Molecular Endocrinology 2007 Aug; 39(2):119.
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PRAS40 is a target for mammalian target of rapamycin complex 1 and is required for signaling downstream of this complex.
Fonseca BD, Smith EM, Lee VH, MacKintosh C, Proud CG.
The Journal of Biological Chemistry 2007 Jun; 282(34):24514.
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Carcinogen-specific gene expression profiles in short-term treated Eker and wild-type rats indicative of pathways involved in renal tumorigenesis.
Stemmer K, Ellinger-Ziegelbauer H, Ahr HJ, Dietrich DR.
Cancer Research 2007 May; 67(9):4052.
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Gender difference of androgen actions on skeletal muscle transcriptome.
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