AKT1S1 recombinant monoclonal antibody, clone R05-3A5
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: HeLa whole cell lysate, Lane 2: A549 whole cell lysate and Lane 3: C6 whole cell lysate with AKT1S1 recombinant monoclonal antibody, clone R05-3A5 (Cat # RAB04463).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human AKT1S1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human AKT1S1.
Theoretical MW (kDa)
Calculated MW: 27 kD
Reactivity
Human, Rat
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
Immunoprecipitation (1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50mM Tris-Glycine, 150mM NaCl, pH 7.4 (40% glycerol, 0.05% BSA and 0.01% Sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: HeLa whole cell lysate, Lane 2: A549 whole cell lysate and Lane 3: C6 whole cell lysate with AKT1S1 recombinant monoclonal antibody, clone R05-3A5 (Cat # RAB04463).Immunoprecipitation
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Gene Info — AKT1S1
Entrez GeneID
84335Protein Accession#
Q96B36Gene Name
AKT1S1
Gene Alias
Lobe, MGC2865, PRAS40
Gene Description
AKT1 substrate 1 (proline-rich)
Omim ID
610221Gene Ontology
HyperlinkGene Summary
AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[supplied by OMIM
Other Designations
OTTHUMP00000196757|OTTHUMP00000196758|OTTHUMP00000196759|OTTHUMP00000196760|proline-rich Akt substrate, 40 kDa
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