UBE2S polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Cell lysate)
UBE2S polyclonal antibody (Cat # PAB1701) is used in Western blot to detect UBE2S in Jurkat cell lysate.
Western Blot (Transfected lysate)
Western blot analysis of UBE2S (arrow) using UBE2S polyclonal antibody (Cat # PAB1701). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the UBE2S gene (Lane 2) (Origene Technologies).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with UBE2S polyclonal antibody (Cat # PAB1701) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
-
Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of UBE2S.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human UBE2S.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
UBE2S polyclonal antibody (Cat # PAB1701) is used in Western blot to detect UBE2S in Jurkat cell lysate.Western Blot (Transfected lysate)
Western blot analysis of UBE2S (arrow) using UBE2S polyclonal antibody (Cat # PAB1701). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the UBE2S gene (Lane 2) (Origene Technologies).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with UBE2S polyclonal antibody (Cat # PAB1701) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma. -
Gene Info — UBE2S
Entrez GeneID
27338Protein Accession#
NP_055316;Q16763Gene Name
UBE2S
Gene Alias
E2-EPF, E2EPF, EPF5
Gene Description
ubiquitin-conjugating enzyme E2S
Omim ID
610309Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the ubiquitin-conjugating enzyme family. The encoded protein is able to form a thiol ester linkage with ubiquitin in a ubiquitin activating enzyme-dependent manner, a characteristic property of ubiquitin carrier proteins. [provided by RefSeq
Other Designations
ubiquitin carrier protein S|ubiquitin-conjugating enzyme E2-24 kD|ubiquitin-protein ligase S
-
Interactome
-
Pathway
-
Publication Reference
-
The Spindle Assembly Checkpoint Is Not Essential for Viability of Human Cells with Genetically Lowered APC/C Activity.
Wild T, Larsen MSY, Narita T, Schou J, Nilsson J, Choudhary C.
Cell Reports 2016 Mar; 14(8):1829.
Application：WB, Human, HCT116 cells.
-
Efficient APC/C substrate degradation in cells undergoing mitotic exit depends on K11 ubiquitin linkages.
Min M, Mevissen TE, De Luca M, Komander D, Lindon C.
Molecular Biology of the Cell 2015 Dec; 26(24):4325.
Application：WB, Human, U2OS cells.
-
UBE2S elongates ubiquitin chains on APC/C substrates to promote mitotic exit.
Garnett MJ, Mansfeld J, Godwin C, Matsusaka T, Wu J, Russell P, Pines J, Venkitaraman AR.
Nature Cell Biology 2009 Nov; 11(11):1363.
Application：WB, Mouse, 3T3-L1 cells.
-
The Spindle Assembly Checkpoint Is Not Essential for Viability of Human Cells with Genetically Lowered APC/C Activity.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com