UBE2S monoclonal antibody, clone AT2C12
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: 293T cell lysate, Lane 2: UBE2S Transfected 293T cell lysate.
Immunofluorescence
Immunofluorescence of human HeLa cells stained with Phalloidin-TRITC (Red) for Actin staining and UBE2S monoclonal antibody, clone AT2C12 (Cat # MAB7885) (1 : 500) with Alexa 488 (Green).
Flow Cytometry
Flow cytometric analysis of Jurkat cell line, staining at 2-5 ug for 1x106cell (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant UBE2S.
Immunogen
Recombinant protein corresponding to amino acids 1-222 of human UBE2S.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG2a, kappa
Recommend Usage
ELISA
Flow Cytometry
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: 293T cell lysate, Lane 2: UBE2S Transfected 293T cell lysate.Immunocytochemistry
Immunofluorescence
Immunofluorescence of human HeLa cells stained with Phalloidin-TRITC (Red) for Actin staining and UBE2S monoclonal antibody, clone AT2C12 (Cat # MAB7885) (1 : 500) with Alexa 488 (Green).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of Jurkat cell line, staining at 2-5 ug for 1x106cell (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line). -
Gene Info — UBE2S
Entrez GeneID
27338Protein Accession#
NP_055316.2Gene Name
UBE2S
Gene Alias
E2-EPF, E2EPF, EPF5
Gene Description
ubiquitin-conjugating enzyme E2S
Omim ID
610309Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the ubiquitin-conjugating enzyme family. The encoded protein is able to form a thiol ester linkage with ubiquitin in a ubiquitin activating enzyme-dependent manner, a characteristic property of ubiquitin carrier proteins. [provided by RefSeq
Other Designations
ubiquitin carrier protein S|ubiquitin-conjugating enzyme E2-24 kD|ubiquitin-protein ligase S
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Interactome
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Pathway
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Publication Reference
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Building ubiquitin chains: E2 enzymes at work.
Ye Y, Rape M.
Nature Reviews. Molecular Cell Biology 2009 Nov; 10(11):755.
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Two different classes of E2 ubiquitin-conjugating enzymes are required for the mono-ubiquitination of proteins and elongation by polyubiquitin chains with a specific topology.
Windheim M, Peggie M, Cohen P.
The Biochemical Journal 2008 Feb; 409(3):723.
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Building ubiquitin chains: E2 enzymes at work.
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