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Last updated: 2016/12/4
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HIST1H3E (phospho S28) polyclonal antibody

  • Catalog # : PAB16952
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of HIST1H3E.
  • Immunogen:
  • Synthetic phosphopeptide corresponding to residues surrounding S28 of human HIST1H3E.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse, Rat
  • Specificity:
  • This antibody detects endogenous levels of HIST1H3E only when phosphorylated at serine 28.
  • Form:
  • Liquid
  • Recommend Usage:
  • Western Blot (1:500-1:1000)
    Immunohistochemistry (1:50-1:100)
    ELISA (1:4000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM PBS, 0.15 M NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C for three months. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of extracts from HUVEC cells treated with Serum.
    Lane 1 : Using HIST1H3E (phospho S28) polyclonal antibody (Cat # PAB16952).
    Lane 2 : Using the same antibody preincubated with synthesized peptide.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human malignant lymphoma tissue using HIST1H3E (phospho S28) polyclonal antibody (Cat # PAB16952).
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 8353
  • Gene Name:
  • HIST1H3E
  • Gene Alias:
  • H3.1,H3/d,H3FD
  • Gene Description:
  • histone cluster 1, H3e
  • Gene Summary:
  • Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq
  • Other Designations:
  • H3 histone family, member D,histone 1, H3e
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