Rabbit polyclonal antibody raised against synthetic peptide of PDK1.
A synthetic peptide corresponding to amino acids 300-400 of human PDK1.
This antibody reacts with human. No other species have been tested. The immunogen used for this antibody production has 100% homology with mouse, rat, and monkey PDK1 protein. This is specific to PDK1. By Western blot a band is seen at ~49 KDa, representing PDK1 protein. There are also two higher and one lower non-specific molecular weight bands.
Western Blot (0.5-2.0 ug/ml) The optimal working dilution should be determined by the end user.
Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. [provided by RefSeq