PDK1 (phospho S241) monoclonal antibody, clone F7 (FITC)
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More Files
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Flow Cytometry
Flow cytometric analysis of 293T cells with PDK1 (phospho S241) monoclonal antibody, clone F7 (FITC) (Cat # MAB21630). Unstained K252a treated cells (blue) or treated with K252a (red) or with pervanadate (green).
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic phosphopeptide of human PDK1.
Immunogen
A synthetic phosphopeptide corresponding to residues surrounding S241 of human PDK1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Conjugation
FITC
Purification
Protein A/G purification
Isotype
IgG1, kappa
Recommend Usage
Flow Cytometry (5 uL/106 cells)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (0.2% BSA, 0.09% sodium azide).
Storage Instruction
Store at 4°C.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Flow Cytometry
Flow cytometric analysis of 293T cells with PDK1 (phospho S241) monoclonal antibody, clone F7 (FITC) (Cat # MAB21630). Unstained K252a treated cells (blue) or treated with K252a (red) or with pervanadate (green). -
Gene Info — PDK1
Entrez GeneID
5163Protein Accession#
Q15118Gene Name
PDK1
Gene Alias
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Gene Description
pyruvate dehydrogenase kinase, isozyme 1
Omim ID
602524Gene Ontology
HyperlinkGene Summary
Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. [provided by RefSeq
Other Designations
mitochondrial pyruvate dehydrogenase kinase isoenzyme 1|pyruvate dehydrogenase kinase, isoenzyme 1
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Interactome
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Pathway
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Disease
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