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Last updated: 2017/1/15
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PAX8 polyclonal antibody

  • Catalog # : PAB14858
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of PAX8.
  • Immunogen:
  • A synthetic peptide corresponding to internal domain of human PAX8.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 48
  • Reactivity:
  • Human, Mouse, Rat
  • Specificity:
  • This antibody recognizes ~48 KDa of human PAX8.
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • Western Blot (0.1-1 ug/mL)
    ELISA (0.01-0.1 ug/mL)
    Immunoprecipitation (2-5 ug/mL)
    Immunohistochemistry (2-5 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.2 (BSA, 10% Proclin300)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C or lower.
    Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • The cell lysate derived from Jurkat was immunoblotted by PAX8 polyclonal antibody (Cat # PAB14858) at 1 : 500. An immunoreactive band is observed at 48 kDa. This band is depleted by immunizing peptide.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • The human breast carcinoma stained with PAX8 polyclonal antibody (Cat # PAB14858) at 1 : 250. The formalin-fixed, paraffin-embedded sections need 15 minutes heat-induced epitope retrieval in 10 mM citrate buffer, pH 6.0, and 30 minutes incubation at room temperature with the primary antibody.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 7849
  • Gene Name:
  • PAX8
  • Gene Alias:
  • -
  • Gene Description:
  • paired box 8
  • Gene Summary:
  • This gene encodes a member of the paired box (PAX) family of transcription factors. Members of this gene family typically encode proteins that contain a paired box domain, an octapeptide, and a paired-type homeodomain. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas and atypical follicular thyroid adenomas. Alternateively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000158659,OTTHUMP00000158660,paired domain gene 8
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