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Last updated: 2022/5/22
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IL18 polyclonal antibody 

  • Catalog # : PAB14391
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of IL18.
  • Immunogen:
  • A synthetic peptide corresponding to human IL18.
  • Sequence:
  • C-NPPDNIKDTKSDI
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 22.3
  • Reactivity:
  • Human
  • Specificity:
  • Approx 23 KDa band observed in human placenta lysates and in lysates of cell line HeLa (calculated MW of 22.3 KDa according to NP_001553.1).
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Recommend Usage:
  • ELISA (1:32000)
    Immunofluorescence (10 ug/mL)
    Flow Cytometry (10 ug/mL)
    Western Blot (1-3 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • IL18 polyclonal antibody (Cat # PAB14391) (0.1 ug/mL) staining of HeLa (1), (2ug/ml) A431 (2) and US0S (3) cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
  • Immunofluorescence
  • Immunofluorescence
  • IL18 polyclonal antibody (Cat # PAB14391) Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing Golgi and cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
  • Immunofluorescence
  • Immunofluorescence
  • IL18 polyclonal antibody (Cat # PAB14391) Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing Golgi and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
  • Enzyme-linked Immunoabsorbent Assay
  • Flow Cytometry
  • Flow Cytometry
  • IL18 polyclonal antibody (Cat # PAB14391) Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Enzyme-linked Immunoabsorbent Assay
  • Gene Information
  • Entrez GeneID:
  • 3606
  • Gene Name:
  • IL18
  • Gene Alias:
  • IGIF,IL-18,IL-1g,IL1F4,MGC12320
  • Gene Description:
  • interleukin 18 (interferon-gamma-inducing factor)
  • Gene Summary:
  • The protein encoded by this gene is a proinflammatory cytokine. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. [provided by RefSeq
  • Other Designations:
  • IL-1 gamma,interferon-gamma-inducing factor,interleukin 18,interleukin-1 gamma,interleukin-18
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