IL18 recombinant monoclonal antibody, clone SAIC-24B-4
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human IL18.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against peptide "ISTLSCENK" derived from IL-18.
Reactivity
Human
Form
Liquid
Isotype
IgG
Recommend Usage
Flow Cytometry
Immuno-MRM (multiple reaction monitoring)
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% Proclin 300)
Storage Instruction
Store at 4°C for 3 months. For long term storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Western blot analysis of Lane 1: human peripheral blood monocyte (untreated) and Lane 2: human peripheral blood monocyte (treated with PMA/lonomycin) with IL18 recombinant monoclonal antibody, clone SAIC-24B-4 (Cat # RAB03572).
Western Blot analysis the lysates (35ug protein in RIPA buffer) were resolved on a 15% SDS PAGE gel and blots were probed with the chimeric r version of RAB03572 at 0.3 ug/mL before detection using an anti-rondary antibody. Protein was detected by chemiluminescence.Flow Cytometry
Flow cytometric analysis of human peripheral blood monocytes with IL18 recombinant monoclonal antibody, clone SAIC-24B-4 (Cat # RAB03572).
The human peripheral blood monocytes were fixed using 2% PFA and permeabilized 0.5% Triton and stained with unimmunized r antibody antibody (isotype control-black line) or the chimeric r version of RAB03572 (blue line) at a dilution of 1:100 for 1h at RT. An additional population of PBMC's stimulated with PMA and Ionomycin in the presence of Monensin for 4 hours were stained in a similar manner (purple line). After washing the bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.Immuno-MRM (multiple reaction monitoring)
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Gene Info — IL18
Entrez GeneID
3606Gene Name
IL18
Gene Alias
IGIF, IL-18, IL-1g, IL1F4, MGC12320
Gene Description
interleukin 18 (interferon-gamma-inducing factor)
Omim ID
600953Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a proinflammatory cytokine. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. [provided by RefSeq
Other Designations
IL-1 gamma|interferon-gamma-inducing factor|interleukin 18|interleukin-1 gamma|interleukin-18
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