PPP4C polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Cell lysate)
PPP4C polyclonal antibody (Cat # PAB14146) (0.3 ug/mL) staining of HEK293 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
PPP4C polyclonal antibody (Cat # PAB14146) (2.5 ug/mL) staining of paraffin embedded human prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
-
Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of PPP4C.
Immunogen
A synthetic peptide corresponding to human PPP4C.
Sequence
C-PQETRGIPSKKP
Host
Goat
Theoretical MW (kDa)
35.1
Reactivity
Human
Specificity
Approx 35 KDa band observed in HEK293, A-431 and HeLa lysates (calculated MW of 35.1 KDa according to NP_002711.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:16000)
Western Blot (0.1-0.3 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
PPP4C polyclonal antibody (Cat # PAB14146) (0.3 ug/mL) staining of HEK293 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
PPP4C polyclonal antibody (Cat # PAB14146) (2.5 ug/mL) staining of paraffin embedded human prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
-
Gene Info — PPP4C
Entrez GeneID
5531Protein Accession#
NP_002711.1Gene Name
PPP4C
Gene Alias
PP4, PPH3, PPX
Gene Description
protein phosphatase 4 (formerly X), catalytic subunit
Omim ID
602035Gene Ontology
HyperlinkGene Summary
O
Other Designations
Protein phosphatase 4, catalytic subunit
-
Interactome
-
Disease
-
Publication Reference
-
Greatwall-phosphorylated Endosulfine is both an inhibitor and a substrate of PP2A-B55 heterotrimers.
Williams BC, Filter JJ, Blake-Hodek KA, Wadzinski BE, Fuda NJ, Shalloway D, Goldberg ML.
ELife 2014 Mar; 3:e01695.
Application：WB-Tr, Drosophila, S2 cells.
-
Protein phosphatase 4 regulates apoptosis, proliferation and mutation rate of human cells.
Mourtada-Maarabouni M, Williams GT.
Biochimica et Biophysica Acta 2008 Aug; 1783(8):1490.
Application：WB-Tr, Human, HEK 293T cells.
-
Greatwall-phosphorylated Endosulfine is both an inhibitor and a substrate of PP2A-B55 heterotrimers.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com