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PHF8 polyclonal antibody

  • Catalog # : PAB14120
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of PHF8.
  • Immunogen:
  • A mixture of synthetic peptides (conjugated with KLH) that corresponding to human PHF8.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse
  • Form:
  • Liquid
  • Recommend Usage:
  • ELISA (1:100-1:500)
    Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.05% sodium azide, 0.05% proclin 300)
  • Storage Instruction:
  • Store at -20°C. For long term storage store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • E14TG2A (mouse embryonic stem cells) were transfected with a conditional allele of PHF8.
    Nuclear extracts (135 ug) from wild type cells (WT) and from 2 clones (F1, B8) with an active and a targeted allele (F1ko, B8ko), respectively, were analysed by Western blot using PHF8 polyclonal antibody (Cat # PAB14120).
    The antibody was diluted 1 : 1,000.
  • ELISA
  • ELISA
  • ELISA was performed using a serial dilution of PHF8 polyclonal antibody (Cat # PAB14120), crude serum and Flow Through in antigen coated wells.
    By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be 1 : 6,500.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Gene Information
  • Gene Name:
  • PHF8
  • Gene Alias:
  • DKFZp686E0868,JHDM1F,KIAA1111,MRXSSD,ZNF422
  • Gene Description:
  • PHD finger protein 8
  • Other Designations:
  • OTTHUMP00000061869,jumonji C domain-containing histone demethylase 1F
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