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Last updated: 2016/10/23
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SOX4 polyclonal antibody

  • Catalog # : PAB14092
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of SOX4.
  • Immunogen:
  • A mixture of synthetic peptides (conjugated with KLH) corresponding to N-terminus and to C-terminus of human SOX4.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Recommend Usage:
  • ELISA (1:100-1:1000)
    Western Blot (1:4000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In serum (0.05% sodium azide)
  • Storage Instruction:
  • Store at -20°C. For long term storage store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • H1299 (lung carcinoma cells) were transfected with HA-tagged SOX4 (Lane 1) or with a HA-tagged truncated SOX4 (395X mutant, Lane 2).
    Whole cell extracts (60~100 ug) were analysed by Western blot using SOX4 polyclonal antibody (Cat # PAB14092) diluted 1 : 4,000 in TBS-Tween containing 2% skimmed milk.
  • Western Blot
  • Western Blot
  • Whole cell extracts of 4 different lung carcinoma cell lines were analysed by Western blot with SOX4 polyclonal antibody (Cat # PAB14092) as described above.
    The H522 cells are known to overexpress SOX4.
    Western blot performed by Montse Sanchez-Cespedes and Sandra Castillo Diez, Catalan Institute of Oncology, Barcelona, Spain.
  • ELISA was performed using a serial dilution of SOX4 polyclonal antibody (Cat # PAB14092). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1 : 18,000.
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 6659
  • Gene Name:
  • SOX4
  • Gene Alias:
  • EVI16
  • Gene Description:
  • SRY (sex determining region Y)-box 4
  • Gene Summary:
  • This intronless gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins, such as syndecan binding protein (syntenin). The protein may function in the apoptosis pathway leading to cell death as well as to tumorigenesis and may mediate downstream effects of parathyroid hormone (PTH) and PTH-related protein (PTHrP) in bone development. The solution structure has been resolved for the HMG-box of a similar mouse protein. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000039358,SRY-related HMG-box gene 4,ecotropic viral integration site 16
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