H1299 (lung carcinoma cells) were transfected with HA-tagged SOX4 (Lane 1) or with a HA-tagged truncated SOX4 (395X mutant, Lane 2). Whole cell extracts (60~100 ug) were analysed by Western blot using SOX4 polyclonal antibody (Cat # PAB14092) diluted 1 : 4,000 in TBS-Tween containing 2% skimmed milk.
Whole cell extracts of 4 different lung carcinoma cell lines were analysed by Western blot with SOX4 polyclonal antibody (Cat # PAB14092) as described above. The H522 cells are known to overexpress SOX4. Western blot performed by Montse Sanchez-Cespedes and Sandra Castillo Diez, Catalan Institute of Oncology, Barcelona, Spain.
ELISA was performed using a serial dilution of SOX4 polyclonal antibody (Cat # PAB14092). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1 : 18,000.
This intronless gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins, such as syndecan binding protein (syntenin). The protein may function in the apoptosis pathway leading to cell death as well as to tumorigenesis and may mediate downstream effects of parathyroid hormone (PTH) and PTH-related protein (PTHrP) in bone development. The solution structure has been resolved for the HMG-box of a similar mouse protein. [provided by RefSeq
OTTHUMP00000039358,SRY-related HMG-box gene 4,ecotropic viral integration site 16