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Last updated: 2017/11/19
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MBD3 polyclonal antibody

  • Catalog # : PAB14055
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of MBD3.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to human MBD3.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Recommend Usage:
  • ELISA (1:100-1:500)
    Western Blot (1:2 000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In serum (0.05% sodium azide)
  • Storage Instruction:
  • Store at -20°C. For long term storage store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot was performed on nuclear extracts from a Human leukemic monocyte lymphoma cell line (U-937 NE, 60 ug) with MBD3 polyclonal antibody (Cat # PAB14055), diluted 1 : 2,000 in TBS-Tween containing 5% skimmed milk.
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • TY1-tagged MBD3 (TTE-MBD3) was over-expressed in osteosarcoma cells (U-2 OS).
    Nuclear extracts were prepared and separated by electrophoresis.
    An antibody directed against the TY1 tag (Lane 1) or crude serum (Lane 2) MBD3 polyclonal antibody (Cat # PAB14055) (diluted 1 : 2,000 in TBS-Tween containing 5% skimmed milk) were used to detect TTE-MBD3.
  • ELISA
  • ELISA
  • ELISA was performed using a serial dilution of MBD3 polyclonal antibody (Cat # PAB14055) in antigen coated wells. By plotting the absorbance against the crude serum dilution, the titer of the antibody was estimated to be 1 : 13,890.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Gene Information
  • Gene Name:
  • MBD3
  • Gene Alias:
  • -
  • Gene Description:
  • methyl-CpG binding domain protein 3
  • Gene Summary:
  • DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). However, unlike the other family members, MBD3 is not capable of binding to methylated DNA. The predicted MBD3 protein shares 71% and 94% identity with MBD2 (isoform 1) and mouse Mbd3. MBD3 is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. MBD3 mediates the association of metastasis-associated protein 2 (MTA2) with the core histone deacetylase complex. [provided by RefSeq
  • Other Designations:
  • -
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