MBD3 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot was performed on nuclear extracts from a Human leukemic monocyte lymphoma cell line (U-937 NE, 60 ug) with MBD3 polyclonal antibody (Cat # PAB14055), diluted 1 : 2,000 in TBS-Tween containing 5% skimmed milk.
Western Blot (Transfected lysate)
TY1-tagged MBD3 (TTE-MBD3) was over-expressed in osteosarcoma cells (U-2 OS).
Nuclear extracts were prepared and separated by electrophoresis.
An antibody directed against the TY1 tag (Lane 1) or crude serum (Lane 2) MBD3 polyclonal antibody (Cat # PAB14055) (diluted 1 : 2,000 in TBS-Tween containing 5% skimmed milk) were used to detect TTE-MBD3.
Enzyme-linked Immunoabsorbent Assay
ELISA was performed using a serial dilution of MBD3 polyclonal antibody (Cat # PAB14055) in antigen coated wells. By plotting the absorbance against the crude serum dilution, the titer of the antibody was estimated to be 1 : 13,890.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MBD3.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to human MBD3.
Host
Rabbit
Reactivity
Human
Form
Liquid
Recommend Usage
ELISA (1:100-1:500)
Western Blot (1:2 000)
The optimal working dilution should be determined by the end user.Storage Buffer
In serum (0.05% sodium azide)
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot was performed on nuclear extracts from a Human leukemic monocyte lymphoma cell line (U-937 NE, 60 ug) with MBD3 polyclonal antibody (Cat # PAB14055), diluted 1 : 2,000 in TBS-Tween containing 5% skimmed milk.Western Blot (Transfected lysate)
TY1-tagged MBD3 (TTE-MBD3) was over-expressed in osteosarcoma cells (U-2 OS).
Nuclear extracts were prepared and separated by electrophoresis.
An antibody directed against the TY1 tag (Lane 1) or crude serum (Lane 2) MBD3 polyclonal antibody (Cat # PAB14055) (diluted 1 : 2,000 in TBS-Tween containing 5% skimmed milk) were used to detect TTE-MBD3.Enzyme-linked Immunoabsorbent Assay
ELISA was performed using a serial dilution of MBD3 polyclonal antibody (Cat # PAB14055) in antigen coated wells. By plotting the absorbance against the crude serum dilution, the titer of the antibody was estimated to be 1 : 13,890. -
Gene Info — MBD3
Entrez GeneID
53615Protein Accession#
O95983Gene Name
MBD3
Gene Alias
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Gene Description
methyl-CpG binding domain protein 3
Omim ID
603573Gene Ontology
HyperlinkGene Summary
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). However, unlike the other family members, MBD3 is not capable of binding to methylated DNA. The predicted MBD3 protein shares 71% and 94% identity with MBD2 (isoform 1) and mouse Mbd3. MBD3 is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. MBD3 mediates the association of metastasis-associated protein 2 (MTA2) with the core histone deacetylase complex. [provided by RefSeq
Other Designations
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