DFFB polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of DFFB in Jurkat cell lysate with DFFB polyclonal antibody (Cat # PAB13404) at (A) 0.5, (B) 1 and (C) 2 ug/mL .
Immunocytochemistry
Immunocytochemistry of DFFB in K-562 cells with DFFB polyclonal antibody (Cat # PAB13404) at 10 ug/mL .
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of DFFB.
Immunogen
A synthetic peptide corresponding to amino acids 203-218 of human DFFB.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
It is human, mouse, and rat reactive
Form
Liquid
Recommend Usage
Western Blot (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of DFFB in Jurkat cell lysate with DFFB polyclonal antibody (Cat # PAB13404) at (A) 0.5, (B) 1 and (C) 2 ug/mL .Immunocytochemistry
Immunocytochemistry of DFFB in K-562 cells with DFFB polyclonal antibody (Cat # PAB13404) at 10 ug/mL . -
Gene Info — DFFB
Entrez GeneID
1677Protein Accession#
NP_004393Gene Name
DFFB
Gene Alias
CAD, CPAN, DFF-40, DFF2, DFF40
Gene Description
DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase)
Omim ID
601883Gene Ontology
HyperlinkGene Summary
Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of these variants has not been determined. [provided by RefSeq
Other Designations
DNA fragmentation factor, 40 kD, beta polypeptide|DNA fragmentation factor, 40 kD, beta polypeptide (caspase-activated DNase)|DNA fragmentation factor, 40 kD, beta subunit|OTTHUMP00000003633|caspase-activated deoxyribonuclease|caspase-activated nuclease
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Interactome
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Pathway
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Publication Reference
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The 40-kDa subunit of DNA fragmentation factor induces DNA fragmentation and chromatin condensation during apoptosis.
Liu X, Li P, Widlak P, Zou H, Luo X, Garrard WT, Wang X.
PNAS 1998 Jul; 95(15):8461.
Application：WB-Tr, Human, HEK 293 cells.
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CPAN, a human nuclease regulated by the caspase-sensitive inhibitor DFF45.
Halenbeck R, MacDonald H, Roulston A, Chen TT, Conroy L, Williams LT.
Current Biology 1998 Apr; 8(9):537.
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A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD.
Enari M, Sakahira H, Yokoyama H, Okawa K, Iwamatsu A, Nagata S.
Nature 1998 Jan; 391(6662):43.
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The 40-kDa subunit of DNA fragmentation factor induces DNA fragmentation and chromatin condensation during apoptosis.
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