NLRC4 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of NLRC4 in human PBL lysate with NLRC4 polyclonal antibody (Cat # PAB12895) at 0.5 (lane A), 1 (lane B), and 2 (lane C) ug/mL , respectively.
Immunocytochemistry
Immunocytochemistry of NLRC4 in THP-1 cells with NLRC4 polyclonal antibody (Cat # PAB12895) at 10 ug/mL .
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of NLRC4.
Immunogen
A synthetic peptide corresponding to C-terminus of human NLRC4.
Host
Rabbit
Reactivity
Human
Specificity
A 110 KDa band can be detected. Ipaf antibody is human specific.
Form
Liquid
Recommend Usage
Western Blot (0.5-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of NLRC4 in human PBL lysate with NLRC4 polyclonal antibody (Cat # PAB12895) at 0.5 (lane A), 1 (lane B), and 2 (lane C) ug/mL , respectively.Immunocytochemistry
Immunocytochemistry of NLRC4 in THP-1 cells with NLRC4 polyclonal antibody (Cat # PAB12895) at 10 ug/mL . -
Gene Info — NLRC4
Entrez GeneID
58484Protein Accession#
NP_067032Gene Name
NLRC4
Gene Alias
CARD12, CLAN, CLAN1, CLANA, CLANB, CLANC, CLAND, CLR2.1, IPAF
Gene Description
NLR family, CARD domain containing 4
Omim ID
606831Gene Ontology
HyperlinkGene Summary
In C. elegans, Ced4 binds and activates Ced3, an apoptotic initiator caspase, via caspase-associated recruitment domains (CARDs). Human Ced4 homologs include APAF1 (MIM 602233), NOD1/CARD4 (MIM 605980), and NOD2/CARD15 (MIM 605956). These proteins have at least 1 N-terminal CARD domain followed by a centrally located nucleotide-binding domain (NBD or NACHT) and a C-terminal regulatory domain, found only in mammals, that contains either WD40 repeats or leucine-rich repeats (LRRs). CARD12 is a member of the Ced4 family and can induce apoptosis.[supplied by OMIM
Other Designations
ICE-protease activating factor|NOD-like receptor C4|caspase recruitment domain family, member 12|caspase recruitment domain protein 12|nucleotide-binding oligomerization domain, leucine rich repeat and CARD domain containing 4
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Interactome
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Disease
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Publication Reference
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Identification of Ipaf, a human caspase-1-activating protein related to Apaf-1.
Poyet JL, Srinivasula SM, Tnani M, Razmara M, Fernandes-Alnemri T, Alnemri ES.
The Journal of Biological Chemistry 2001 Jul; 276(30):28309.
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Human CARD12 is a novel CED4/Apaf-1 family member that induces apoptosis.
Geddes BJ, Wang L, Huang WJ, Lavellee M, Manji GA, Brown M, Jurman M, Cao J, Morgenstern J, Merriam S, Glucksmann MA, DiStefano PS, Bertin J.
Biochemical and Biophysical Research Communications 2001 Jun; 284(1):77.
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Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade.
Li P, Nijhawan D, Budihardjo I, Srinivasula SM, Ahmad M, Alnemri ES, Wang X.
Cell 1997 Nov; 91(4):479.
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Identification of Ipaf, a human caspase-1-activating protein related to Apaf-1.
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